Luminex Case Study: University of North Texas & xMAP® Technology

High-speed, highly-multiplexed assays for breakthrough research



The FLEXMAP 3D® is Luminex’s most advanced and versatile multiplexing platform. Fast read time, compatibility with both 96- and 384-well plates, and ease of integration with front-end automation systems make the FLEXMAP 3D the platform of choice for high throughput applications. The platform uses both magnetic and non-magnetic microspheres and is capable of simultaneously measuring up to 500 genes or proteins from a small sample. Automated start up, shut down, and maintenance routines make the FLEXMAP 3D easy to use and maintain.



Key Features:


  • Multiplexing: Up to 500 analytes in a single sample
  • Dynamic Range (Typical): ≥4.5 logs
  • Read Time: 96-well plate in ~20 min (up to 144,000 tests/hour); 384-well plate in ~75 min (up to 153,600 tests/hour)
  • Daily Start-Up/Shut-Down: ~30 min



Let FLEXMAP 3D empower your research with:


  • Higher Throughput: Dual sample fluidics paths and increased sample injection speed result in a faster sampling rate. The FLEXMAP 3D system can process 384 samples and generate up to 144,000 data points in just over an hour.
  • High Plexing Capability: The FLEXMAP 3D is compatible with magnetic and non-magnetic microspheres and can discriminate up to 500 different microsphere sets. The platform offers a large menu of commercial kits and reagents to develop custom assays for a wide variety of applications including nucleic acid assays, immunoassays, receptor ligand assays, and enzymatic assays.
  • Automation/LIS Compatibility: xPONENT® Software controls the FLEXMAP 3D system and offers interfacing options for Laboratory Information Systems (LIS) and front-end automation platforms. The system accommodates 96 and 384-well plate formats for enhanced sample flexibility and increased throughput.
  • Reliability: The FLEXMAP 3D system has multiple features allowing walk away start-up, shutdown, and maintenance operations, and greatly enhances usability for High Throughput Screening (HTS) applications.
Multiplexing Capability Up to 500 individual analytes
Dynamic Range ≥ 4.5 logs
Physical Dimensions 58.4 cm W x 63.5 cm D x 54.7 cm H (23” W x 25.7” D x 18” )
Weight No more than 200 lbs (91 Kg)
Plate Heater Operating Range 35 – 60°C (95 – 131°F)
System Warm Up 30 minutes
Sheath Fluid Pressure 8 – 13 psi
Sample Injection Rate Two µl/second
Sample Uptake Volume 10 – 200 µl
Classification Laser Excitation 638 nm
Reporter Laser Excitation 532 nm
Reporter Detector 565 nm – 585 nm
All Luminex instrumentation is marked in compliance with electrical/safety device standards for appropriate regions. For details on approvals and standards compliance please contact Luminex.
IQ/OQ Services
Installation Qualification (IQ) and Operational Qualification (OQ) services are performed by Luminex Field Service Engineers, and are critical to ensuring that your instrument is properly installed and operating according to specifications. For more information, please click here, contact your Luminex representative, or email orders@luminexcorp.com.

FLEXMAP 3D® Reagents


The FLEXMAP 3D® instrument requires sheath fluid to serve as the delivery medium to transport the sample to the instrument’s optics.


This instrument has the option of a 20 L disposable external sheath fluid container or a compact 20X sheath fluid concentrate pack; which can be reconstituted to a 20 L working concentration.


Apart from the significantly reduced storage space required, the concentrate pack can save the user considerable shipping costs; upwards of 80% savings, in some cases.


All xMAP® instruments requires regular calibration and performance verification testing, to ensure the system is operating correctly and to maintain data accuracy. The FLEXMAP 3D instrument is calibrated using a calibration and a verification kit, each providing 25 uses.


Reagent Shipping Policy Information


Sheath Fluid (20 L) has a two year shelf life from the date of manufacture, and Luminex guarantees a shelf life of no less than six months from the shipment date.


Sheath Concentrate Pack (1 L) has a nine month shelf life from the date of manufacture, and a guaranteed three month minimum shelf life.


Calibration and Verification reagents all have a two year shelf life and a guaranteed six month minimum shelf life from the shipment date.


All Sheath Fluid, Calibration, and Verification reagents ship within three business days via ground carrier. However, please note that backordered items may have longer than normal lead times.


Click here to review the entire Reagent Shipping Policy.

Sheath Fluid

Sheath Fluid

FLEXMAP 3D® Calibration Kit

FLEXMAP 3D Calibration Kit

FLEXMAP 3D® Performance Verification Kit

FLEXMAP 3D Verification Kit

Ordering Information


Luminex Online Order Management
Email: orders@luminexcorp.com
Phone: 1-512-336-3550
Toll Free: 1-866-401-5450
Fax: 1-512-219-0544
Fax (Europe, Middle East, Asia, India): +31 (0)73 800 1998
Product RUO Part Number IVD Part Number1
Product RUO Part Number IVD Part Number1
FLEXMAP 3D Calibration Kit (25 uses) F3D-CAL-K25 F3DIVD-CAL-K25
FLEXMAP 3D Performance Verification Kit (25 uses) F3D-PVER-K25 F3DIVD-PVER-K25
Sheath Concentrate Pack (20 x 1 L) 40-50018 40-75680
Sheath Fluid (20 L) 40-50015 40-50000
Accessories & Spare Parts
Product Part Number
384-Well Plate Probe Height Adjustment Tool CN-0298-01
Barcode Scanner, USB CN-PC03-01
FLEXMAP 3D Swivel Base CN-0216-01
Fuse (6 AMP) CN-0226-01
Heater Block, 96-Well Microtiter Plate CN-0224-01
Heater Block, 384-Well Microtiter Plate CN-0223-01
Off-Plate Reagent Block CN-0225-01
Sample Probe Height Adjustment Kit CN-0263-01
Sample Probe Needle CN-0221-01
Syringe, 500 mL with Seal CN-0013-01
Syringe seal CN-0014-01



FLEXMAP 3DProduct Information

xMAP Technology Products and ServicesCatalog

Instrument Installation and Operational Services (IQ/OQ)Product Information

xMAP® Technology Fact SheetProduct Information

xMAP® KitFinderPartner Kits



General Routine Videos





Maintenance Videos





Troubleshooting Videos


The FLEXMAP 3D system is a class 1(I) laser product.

  1. If using these products for clinical diagnostic applications, ordering the IVD part number will provide reagents with appropriate In Vitro Diagnostic labeling.
    Note: For In Vitro Diagnostic Use. Products are region specific and may not be approved in some countries/regions. Please contact Luminex at support@luminexcorp.com to obtain the appropriate product information for your country of residence.