Salmonellosis is the most frequently reported cause of foodborne illness, causing an estimated 1.4 million cases of foodborne illness and over 400 deaths annually in the US.1
The Luminex xMAP® Salmonella Serotyping Assay (SSA) is a multiplex, nucleic acid-based assay for research or epidemiological use in identifying serotypes greater than 85%2 of the most commonly encountered Salmonella isolates within hours compared with existing methods that can take up to six weeks for results.
|xMAP SSA||Antisera Agglutination|
|Simultaneous identification of 85% of most commonly encountered Salmonella serotypes||Iterations of individual antisera agglutination tests plus phase inversion|
|Can serotype rough and problematic isolates||Cannot serotype rough and non-motile isolates|
|cGMP reagents||Lengthy individual antisera lab validation due to lot to lot inconsistencies|
The xMAP Salmonella Serotyping Assay was developed by the CDC3-4 for fast and comprehensive molecular Salmonella serotyping. The assay consists of three separate tests that detect O and H antigens simultaneously, including the ability to identify some serotype-specific markers in the Additional Targets or AT test.
|Antigen Targets cCovered by xMAP SSA|
|O-Group||B, C1, C2, D, E, G, and serotype Paratyphi A (ParaA)|
|H-Group||a, b, c, d, j, (e,h), i, k, r, z10, z, z29, z6, y, L-complex, v, z28, EN-complex, x, z15, 1-complex, 2, 5, 6, 7, G-complex, f, (m/g,m), (m/m,t), p, s, t-1*, z51, z4-complex, and z24|
|AT||sdf (specific for S. enteritidis), fljB (positive control for second H Antigen phase), and Vi (specific for S. typhi)|
DNA is extracted and quantified from a Salmonella isolate using a simple 15 minute extraction method.
Multiplexed PCR with biotinylated primers is performed for each of the three tests included in the assay.
Labeled amplicon is hybridized to the appropriate oligonucleotide probe-coupled microsphere mixture and labeled with SAPE reporter.
The assay plate is analyzed on a Luminex® 100/200™.
Data generated may be further analyzed via the optional Salmonella Analysis Tool to determine serotype based on the Kauffman-White-Le Minor scheme.
|Products Available from: Luminex & Biovet|
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|Product||Size||Registration Status||Part Number||Available From|
US Public Health6
|xMAP Salmonella Serotyping Assay for Epidemiological Surveillance||100 tests/20 uses||Research Use Only (RUO)||AGSSA4502||Luminex|
|Assay Buffer||40 mL (100 tests)||Research Use Only (RUO)||40-00022||Luminex|
|xMAP Salmonella Serotyping Assay (SSA)5||100 tests/20 uses||Research Use Only (RUO)||AGSSA4502||Biovet|
|Assay Buffer||40 mL (100 tests)||Research Use Only (RUO)||40-00022||Biovet|
xMAP® Salmonella Serotyping Assay – Product Information
Salmonella Analysis Tool – Application
Salmonella Analysis Tool – User Instructions
Salmonella for xPONENT – Instrument Protocols
xPONENT for Salmonella Serotyping Assay – Protocol Set Up Instructions
This product is for Research Use Only, not for use in Diagnostic procedures. Please contact Luminex at firstname.lastname@example.org to obtain the appropriate product information for your country of residence.